Detection of Japanese Yam Mosaic Virus by ELISA and RT-PCR.
نویسندگان
چکیده
منابع مشابه
Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA.
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1999
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.65.207